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ppcr-script cam+ cloning vector  (Agilent technologies)


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    Structured Review

    Agilent technologies ppcr-script cam+ cloning vector
    Ppcr Script Cam+ Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcr-script+cam++cloning+vector/pm37834049-458-2-6
    Average 90 stars, based on 1 article reviews
    ppcr-script cam+ cloning vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Plasmid Preparation:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Purification:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Polymerase Chain Reaction:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Chick Chorioallantoic Membrane Assay:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Transformation Assay:

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells.
    Article Snippet: Into the pPCR-Script Cam+ cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi
    Article Snippet: The PCR-amplified fragment was cloned into the pPCR-Script Cam SK(+) cloning vector (Stratagene).

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-?-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5′CS ( 22 ) and 3′CS ( 12 ) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK(+) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).

    Article Title: Lipopolysaccharide of Legionella pneumophila Serogroup 1 Facilitates Interaction with Host Cells
    Article Snippet: Into the pPCR-Script Cam + cloning vector (Agilent, Santa Clara, CA, USA), the purified PCR product (primers LPS ORF 6a uprc/Corby 7–9 uprc) was inserted at the Srf I blunt site according to the manufacturer’s instruction leading to the plasmid pMPH7_A.

    Article Title: Characterization of In 100 , a New Integron Carrying a Metallo-β-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa
    Article Snippet: The PCR product obtained with the class1 integron primers INT/5 CS (22) and 3 CS (12) was purified with the StrataPrep PCR purification kit (Stratagene, Amsterdam, The Netherlands), and the insert was ligated into the pPCR-Script Cam SK( ) cloning vector (Stratagene, Amsterdam, The Netherlands) and transformed into Epicurian coli XL10-Gold Kan ultracompetent cells (Stratagene, Amsterdam, The Netherlands).



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